Manufactured by:ELK Biotechnology
RUO

Zic1/2/3/4/5 rabbit pAb

Zic1/2/3/4/5 rabbit pAb from ELK Biotechnology

SKU:
ABM109372
Product type
Primary Antibody
TargetZic1/2/3/4/5
ApplicationsIF, ELISA
Species / reactivityHuman, Mouse
Expression systemCNS. A high level expression is seen in the cerebellum. Detected in the nuclei of the cerebellar granule cell lineage from the progenitor cells of the external germinal layer to the postmigrated cells of the internal granular layer. Detected in medulloblastoma (26/29 cases), but not present in all other tumors examined.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Zic1/2/3/4/5 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Zic1/2/3/4/5 in IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development. Aberrant expression of this gene is seen in medulloblastoma, a childhood brain tumor. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 4, a related family member on chromosome 3. This gene encodes a transcription factor that can bind and transactivate the apolipoprotein E gene. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Zic1/2/3/4/5 Polyclonal Antibody detects endogenous levels of Zic1/2/3/4/5 protein.
Validation evidence captions Immunofluorescence analysis of HepG2 cells, using ZIC1/2/3/4/5 Antibody. The picture on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
Antibody Research
Also known as
ZIC1; ZIC; ZNF201; Zinc finger protein ZIC 1; Zinc finger protein 201; Zinc finger protein of the cerebellum 1; ZIC2; Zinc finger protein ZIC 2; Zinc finger protein of the cerebellum 2; ZIC3; ZNF203; Zinc finger protein ZIC 3; Zinc finger p
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human ZIC1/2/3/4/5. AA range:281-330
Cellular localization
Nucleus. Cytoplasm . Localizes in the cytoplasm in presence of MDFIC overexpression. .
Assay type
Antibody validation/application
Source organism
Rabbit
Molecular weight
55kD
Concentration
1 mg/ml
Working concentration
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 281-330
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES7539
UniProt entry
Q15915/O95409/O60481/Q8N9L1/Q96T25
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00