Integrin β1 (phospho Thr789) rabbit pAb

Integrin β1 (phospho Thr789) rabbit pAb

Integrin β1 (phospho Thr789) rabbit pAb
Manufactured by:ELK Biotechnology
RUO

Integrin β1 (phospho Thr789) rabbit pAb

Integrin β1 (phospho Thr789) rabbit pAb from ELK Biotechnology

SKU:
ABM108793
Product type
Primary Antibody
TargetIntegrin β1
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression system[Isoform 1]: Widely expressed, other isoforms are generally coexpressed with a more restricted distribution. ; [Isoform 2]: Expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. ; [Isoform 3]: Together with isoform 4, is expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. ; [Isoform 4]: Together with isoform 3, is expressed in muscle, kidney, liver, placenta, ce
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Integrin β1 (phospho Thr789) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Integrin β1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Phospho-Integrin β1 (T789) Polyclonal Antibody detects endogenous levels of Integrin β1 protein only when phosphorylated at T789.
Validation evidence captions Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Integrin beta1 (Phospho-Thr789) Antibody | Immunofluorescence analysis of COS7 cells, using Integrin beta1 (Phospho-Thr789) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Integrin beta1 (Phospho-Thr789) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HepG2 cells treated with Ca2+ 40uM 30', using Integrin beta1 (Phospho-Thr789) Antibody. The lane on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Rap1 signaling pathway, >>Phagosome, >>PI3K-Akt signaling pathway, >>Axon guidance, >>Focal adhesion, >>ECM-receptor interaction, >>Cell adhesion molecules, >>Tight junction, >>Platelet activation, >>Leukocyte transendothelial migration, >>Regulation of actin cytoskeleton, >>Bacterial invasion of epithelial cells, >>Pathogenic Escherichia coli infection, >>Shigellosis, >>Pertussis, >>Yersinia infection, >>Leishmaniasis, >>Toxoplasmosis, >>Human papillomavirus infection, >>Pathways in cancer, >>Proteoglycans in cancer, >>Small cell lung cancer, >>Hypertrophic cardiomyopathy, >>Arrhythmogenic right ventricular cardiomyopathy, >>Dilated cardiomyopathy
Also known as
ITGB1; FNRB; MDF2; MSK12; Integrin beta-1; Fibronectin receptor subunit beta; VLA-4 subunit beta; CD antigen CD29
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human Integrin beta1 around the phosphorylation site of Thr789. AA range:749-798
Observed band
90kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein . Cell projection, invadopodium membrane ; Single-pass type I membrane protein . Cell projection, ruffle membrane ; Single-pass type I membrane protein . Recycling endosome . Melanosome . Cleavage furrow . Cell projection, lamellipodium . Cell projection, ruffle . Cell junction, focal adhesion . Cell surface . Isoform 2 does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basa
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 749-798
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES5947
UniProt entry
P05556
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00