Manufactured by:ELK Biotechnology
RUO

AT1 rabbit pAb

AT1 rabbit pAb from ELK Biotechnology

SKU:
ABM108408
Product type
Primary Antibody
TargetAGTR1
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemLiver, lung, adrenal and adrenocortical adenomas.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

AT1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying AGTR1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene. [provided by RefSeq, Jul 2012],

Additional antibody information

Specificity AT1 Polyclonal Antibody detects endogenous levels of AT1 protein.
Validation evidence captions Western Blot analysis of HEPG2 cells using AT1 Polyclonal Antibody diluted at 1:500 | Immunofluorescence analysis of MCF7 cells, using AGTR1 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from K562 cells, using AGTR1 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HeLa cells using AGTR1 antibody.
Key facts & specifications
Research areas
>>Calcium signaling pathway, >>cGMP-PKG signaling pathway, >>Phospholipase D signaling pathway, >>Neuroactive ligand-receptor interaction, >>Adrenergic signaling in cardiomyocytes, >>Vascular smooth muscle contraction, >>Apelin signaling pathway, >>Renin-angiotensin system, >>Renin secretion, >>Aldosterone synthesis and secretion, >>Cortisol synthesis and secretion, >>AGE-RAGE signaling pathway in diabetic complications, >>Cushing syndrome, >>Coronavirus disease - COVID-19, >>Pathways in cancer, >>Diabetic cardiomyopathy
Also known as
AGTR1; AGTR1A; AGTR1B; AT2R1; AT2R1B; Type-1 angiotensin II receptor; AT1AR; AT1BR; Angiotensin II type-1 receptor; AT1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human AGTR1. AA range:101-150
Observed band
41kD
Cellular localization
Cell membrane ; Multi-pass membrane protein .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 101-150
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES5032
UniProt entry
P30556
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

AGTR1angiotensin II receptor type 1

View target guide

Angiotensin II receptor type 1 (AGTR1), commonly called the AT1 receptor or AT1R, is a G protein-coupled receptor for angiotensin II and a central signalling component of the renin-angiotensin system. This ABMIUM record links human AGTR1 (NCBI Gene 185; UniProt P30556) to catalogue-backed research products.

Biological function: AGTR1 encodes the type-1 angiotensin II receptor, a G protein-coupled receptor activated by angiotensin II. Curated UniProt evidence describes coupling to Gq-family G proteins, phospholipase C activation and increased cytosolic calcium, supporting angiotensin-II responses involved in vascular tone, blood-pressure regulation and renal sodium handling.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
185
UniProtKB
P30556
ELK Biotechnology
£220.00