Manufactured by:ELK Biotechnology
RUO

Rad GTPase rabbit pAb

Rad GTPase rabbit pAb from ELK Biotechnology

SKU:
ABM107463
Product type
Primary Antibody
TargetRad GTPase
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemMost abundantly expressed in the heart. Also found in the skeletal muscle and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Rad GTPase rabbit pAb is a research-use antibody from ELK Biotechnology for studying Rad GTPase in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

similarity:Belongs to the small GTPase superfamily. RGK family.,subunit:Interacts with calmodulin preferentially to the inactive, GDP-form. Binds CAMKII which is capable of phosphorylating RAD in vitro.,tissue specificity:Skeletal and cardiac muscle, and lung. Lesser amounts in placenta and kidney. Also detected in adipose tissue. Overexpressed in muscle of type II diabetic humans.,

Additional antibody information

Specificity Rad GTPase Polyclonal Antibody detects endogenous levels of Rad GTPase protein.
Validation evidence captions Western Blot analysis of various cells using Rad GTPase Polyclonal Antibody | Immunofluorescence analysis of A549 cells, using RAD Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human heart tissue, using RAD Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HeLa and HepG2/MCF-7 cells, using RAD Antibody. The lane on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
Antibody Research
Also known as
RRAD; RAD; GTP-binding protein RAD; RAD1; Ras associated with diabetes
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human RAD. AA range:41-90
Observed band
33kD
Cellular localization
Cell membrane .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 41-90
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES3302
UniProt entry
P55042
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00