Manufactured by:ELK Biotechnology
RUO

Fusin rabbit pAb

Fusin rabbit pAb from ELK Biotechnology

SKU:
ABM107071
Product type
Primary Antibody
TargetCXCR4
ApplicationsWB, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemExpressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Fusin rabbit pAb is a research-use antibody from ELK Biotechnology for studying CXCR4 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

C-X-C motif chemokine receptor 4(CXCR4) Homo sapiens This gene encodes a CXC chemokine receptor specific for stromal cell-derived factor-1. The protein has 7 transmembrane regions and is located on the cell surface. It acts with the CD4 protein to support HIV entry into cells and is also highly expressed in breast cancer cells. Mutations in this gene have been associated with WHIM (warts, hypogammaglobulinemia, infections, and myelokathexis) syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Fusin Polyclonal Antibody detects endogenous levels of Fusin protein.
Validation evidence captions Western blot analysis of lysates from 1) Hela, 2) mouse-brain cells, (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Tubulin β Monoclonal Antibody(5G3) (Immunoway:YM3030) antibody was diluted at 1:5000 as loading control, 4° over night,Dylight 680 secondary antibody(Immunoway:RS23710)was diluted at 1:10000, 37° 1hour. | Western Blot analysis of various cells using Fusin Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of MOUSE-BRAIN cells using Fusin Polyclonal Antibody diluted at 1:1000 | Immunofluorescence analysis of A549 cells, using CXCR4 Antibody. The picture on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>Viral life cycle - HIV-1, >>Calcium signaling pathway, >>Cytokine-cytokine receptor interaction, >>Viral protein interaction with cytokine and cytokine receptor, >>Chemokine signaling pathway, >>Endocytosis, >>Axon guidance, >>Leukocyte transendothelial migration, >>Intestinal immune network for IgA production, >>Regulation of actin cytoskeleton, >>Human cytomegalovirus infection, >>Human immunodeficiency virus 1 infection, >>Pathways in cancer
Also known as
CXCR4; C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; FB22; Fusin; HM89; LCR1; Leukocyte-derived seven transmembrane domain receptor; LESTR; NPYRL; Stromal cell-derived factor 1 receptor; SDF-1 receptor; CD antigen CD184
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human CXCR4. AA range:300-349
Observed band
36kD
Cellular localization
Cell membrane ; Multi-pass membrane protein . Cell junction. Early endosome. Late endosome. Lysosome. In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation cluster (SMAC).
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 300-349
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES2380
UniProt entry
P61073
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CXCR4C-X-C motif chemokine receptor 4

View target guide

CXCR4 identifies C-X-C motif chemokine receptor 4 in Homo sapiens (Human). This target record connects NCBI Gene 7852, UniProtKB P61073 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
7852
UniProtKB
P61073
ELK Biotechnology
£220.00