CREB-1 (phospho Ser133) rabbit pAb

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Manufactured by:ELK Biotechnology
RUO

CREB-1 (phospho Ser133) rabbit pAb

CREB-1 (phospho Ser133) rabbit pAb from ELK Biotechnology

SKU:
ABM104262
Product type
Primary Antibody
TargetCREB-1
ApplicationsWB, IHC, IF, ELISA, IP
Species / reactivityHuman, Mouse, Rat, Pig
Expression systemEye,Placenta,Spleen,Testis,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

CREB-1 (phospho Ser133) rabbit pAb is a research-use antibody from ELK Biotechnology for studying CREB-1 in WB, IHC, IF, ELISA, IP workflows with listed reactivity for Human, Mouse, Rat, Pig. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016],

Additional antibody information

Specificity Phospho-CREB-1 (S133) Polyclonal Antibody detects endogenous levels of CREB-1 protein only when phosphorylated at S133.
Key facts & specifications
Research areas
>>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>PI3K-Akt signaling pathway, >>AMPK signaling pathway, >>Longevity regulating pathway, >>Adrenergic signaling in cardiomyocytes, >>Osteoclast differentiation, >>Antigen processing and presentation, >>TNF signaling pathway, >>Circadian rhythm, >>Circadian entrainment, >>Thermogenesis, >>Cholinergic synapse, >>Dopaminergic synapse, >>Insulin secretion, >>Estrogen signaling pathway, >>Melanogenesis, >>Thyroid hormone synthesis, >>Glucagon signaling pathway, >>Renin secretion, >>Aldosterone synthesis and secretion, >>Relaxin signaling pathway, >>Cortisol synthesis and secretion, >>Parathyroid hormone synthesis, secretion and action, >>Insulin resistance, >>Cushing syndrome, >>Growth hormone synthesis, >>Vasopressin-regulated water reabsorption, >>Huntington disease, >>Prion disease, >>Cocaine addiction, >>Amphetamine addiction, >>Alcoholism, >>Tuberculosis, >>Hepatitis B, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Human
Also known as
CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser133. AA range:100-149
Observed band
37kD
Cellular localization
Nucleus .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 100-149
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Pig
Identifiers & database references
Supplier SKU / catalogue number
ES1292
UniProt entry
P16220
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00