Manufactured by:ELK Biotechnology
RUO

ANXA5 rabbit pAb

ANXA5 rabbit pAb from ELK Biotechnology

SKU:
ABM103201
Product type
Primary Antibody
TargetANXA5
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemAdipocyte,Epithelium,Lung,Melanoma,Muscle,Ovary,Placenta,Skin,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

ANXA5 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ANXA5 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity ANXA5 Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
Antibody Research
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Immunogen
Synthesized peptide derived from human protein . at AA range: 60-140
Observed band
35kD
Cellular localization
intracellular,cytoplasm,focal adhesion,external side of plasma membrane,membrane,extracellular exosome,endothelial microparticle,
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 60-140
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES11272
UniProt entry
P08758
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ANXA5annexin A5

View target guide

Annexin A5 (ANXA5), widely known as annexin V, is a calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine and established anticoagulant properties.

Biological function: Reviewed UniProt P08758 describes ANXA5 as a calcium-dependent phospholipid-binding protein and indirect inhibitor of the thromboplastin-specific coagulation complex. Its phosphatidylserine-binding behaviour underpins widespread experimental use in membrane asymmetry and cell-death research.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
308
UniProtKB
P08758
ELK Biotechnology
£220.00