Manufactured by:ELK Biotechnology
RUO

ADAM10 rabbit pAb

ADAM10 rabbit pAb from ELK Biotechnology

SKU:
ABM102522
Product type
Primary Antibody
TargetADAM10
ApplicationsWB, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemExpressed in the brain (at protein level) (PubMed:23676497). Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver (PubMed:11511685, PubMed:9016778).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

ADAM10 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ADAM10 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

ADAM metallopeptidase domain 10(ADAM10) Homo sapiens Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. [provided by RefSeq, Feb 2016],

Additional antibody information

Specificity ADAM10 Polyclonal Antibody detects endogenous levels of ADAM10 protein.
Validation evidence captions Western Blot analysis of various cells using ADAM10 Polyclonal Antibody
Key facts & specifications
Research areas
>>Alzheimer disease, >>Epithelial cell signaling in Helicobacter pylori infection
Also known as
ADAM10; KUZ; MADM; Disintegrin and metalloproteinase domain-containing protein 10; ADAM 10; CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; CD antigen CD156c
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
Synthesized peptide derived from ADAM10 . at AA range: 170-250
Observed band
85kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein . Golgi apparatus membrane ; Single-pass type I membrane protein . Cytoplasmic vesicle, clathrin-coated vesicle . Cell projection, axon . Cell projection, dendrite . Cell junction, adherens junction . Cytoplasm . Is localized in the plasma membrane but is also expressed in the Golgi apparatus and in clathrin-coated vesicles derived likely from the Golgi (PubMed:12475894). During long term depression, it is recruited to the cell membrane by DLG1 (PubMed:23676497). The immature form is mainly located near cytoplasmic fibrillar structures, while the mature form is predominantly located at zonula adherens and the cell membrane (PubMed:30463011). The localization and clustering of mature ADAM10 to zonula adherens is regulated by AFDN, TSPAN33,
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 170-250
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES1010
UniProt entry
O14672
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ADAM10ADAM metallopeptidase domain 10

View target guide

ADAM metallopeptidase domain 10 (ADAM10), also known as CD156c, is a membrane-associated disintegrin and metalloprotease. This ABMIUM target record links ADAM10 biology, verified identifiers and catalogue-backed research products.

Biological function: ADAM10 is a cell-surface metalloprotease with disintegrin and protease domains. Curated records describe ectodomain cleavage of multiple membrane proteins, supporting roles in regulated protein shedding, cell adhesion and signalling.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
102
UniProtKB
O14672
ELK Biotechnology
£220.00