Rat CASP3(Caspase 3) ELISA Kit

Rat CASP3(Caspase 3) ELISA Kit - ABMIUM

Rat CASP3(Caspase 3) ELISA Kit - ABMIUM
Manufactured by:Elabscience
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Rat CASP3(Caspase 3) ELISA Kit

Rat CASP3(Caspase 3) ELISA Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM12412-1
Product type
ELISA Kits
TargetCASP3

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Rat CASP3(Caspase 3) ELISA Kit is a research-use ELISA kit from Elabscience for measuring CASP3. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Key facts & specifications
Research areas
Cancer, Cell Biology, Metabolism, Neuroscience
Also known as
Casp3,Cpp32,Caspase-3,CPP-32,IRPLICE,SCA-1,Protein Yama,Caspase 3 subunit p17,Caspase-3 subunit p12
Cross-reactivity
This kit recognizes Rat CASP3 in samples. No significant cross-reactivity or interference between Rat CASP3 and analogues was observed
Incubation temperature
2-8℃
Assay type
Traditional ELISA Kits
Detection method
Colorimetric method,ELISA,Sandwich
Plate format
48-well microplate plates pre-coated with anti-CASP3 capture antibody
Technical note
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CASP3. You can calculate the concentration of Rat CASP3 in the samples by comparing the OD of the samples to the standard curve.
Identifiers & database references
Supplier SKU / catalogue number
E-EL-R0160_48T
Gene symbol
CASP3
Gene ID
25402
UniProt accession
P55213
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CASP3caspase 3

View target guide

CASP3 identifies caspase 3 in Homo sapiens (Human). This target record connects NCBI Gene 836, UniProtKB P42574 and related ABMIUM catalogue products.

Biological function: CASP3 is a major executioner caspase in apoptosis. After proteolytic activation by initiator caspases, it cleaves numerous cellular substrates to carry out the apoptotic programme.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
836
UniProtKB
P42574
Browse all CASP3 productsRead target context & evidence
Elabscience
£363.00£254.10