Human PAPPA(Pregnancy Associated Plasma Protein A) ELISA Kit

Human PAPPA(Pregnancy Associated Plasma Protein A) ELISA Kit - ABMIUM

Human PAPPA(Pregnancy Associated Plasma Protein A) ELISA Kit - ABMIUM
Manufactured by:Elabscience
Save 30%ABMIUM Verified™RUO

Human PAPPA(Pregnancy Associated Plasma Protein A) ELISA Kit

Human PAPPA(Pregnancy Associated Plasma Protein A) ELISA Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM11546-1
Product type
ELISA Kits
TargetPAPPA

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Human PAPPA(Pregnancy Associated Plasma Protein A) ELISA Kit is a research-use ELISA kit from Elabscience for measuring PAPPA. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Key facts & specifications
Research areas
Cell Biology, Cardiovascular
Also known as
PAPPA
Cross-reactivity
This kit recognizes Human PAPPA in samples. No significant cross-reactivity or interference between Human PAPPA and analogues was observed
Incubation temperature
2-8℃
Assay type
Traditional ELISA Kits
Detection method
Colorimetric method,ELISA,Sandwich
Plate format
48-well microplate plates pre-coated with anti-PAPPA capture antibody
Technical note
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAPPA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAPPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAPPA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PAPPA. You can calculate the concentration of Human PAPPA in the samples by comparing the OD of the samples to the standard curve.
Identifiers & database references
Supplier SKU / catalogue number
E-EL-H0145_48T
Gene symbol
PAPPA
Gene ID
5069
UniProt accession
Q13219
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

PAPPApappalysin 1

View target guide

PAPPA identifies pappalysin 1 in Homo sapiens (Human). This target record connects NCBI Gene 5069, UniProtKB Q13219 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
5069
UniProtKB
Q13219
Browse all PAPPA productsRead target context & evidence
Elabscience
£363.00£254.10