Manufactured by:Elabscience
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Rat BAD (BCL-2 Associated Death Promoter) CLIA Kit

Rat BAD (BCL-2 Associated Death Promoter) CLIA Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM11308-1
Product type
Assay Kits
TargetBAD

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Rat BAD (BCL-2 Associated Death Promoter) CLIA Kit from Elabscience is intended for research workflows involving BAD. Review the listed specifications and supporting documents to confirm compatibility with the intended method.

Key facts & specifications
Research areas
Cancer, Cell Biology, Metabolism
Cross-reactivity
This kit recognizes Rat BAD in samples.No significant cross-reactivity or interference between Rat BAD and analogues was observed
Incubation temperature
2-8℃/-20℃
Assay type
CLIA Kits
Detection method
Chemiluminescence
Plate format
48-well microplate plates pre-coated with anti-BAD capture antibody
Technical note
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BAD. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BAD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BAD, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat BAD. You can calculate the concentration of Rat BAD in the samples by comparing the RLU value of the samples to the standard curve.
Identifiers & database references
Supplier SKU / catalogue number
E-CL-R0071_48T
Gene symbol
BAD
Gene ID
64639
UniProt accession
O35147
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

BADBCL2 associated agonist of cell death

View target guide

BCL2 associated agonist of cell death (BAD) is a pro-apoptotic BH3-only BCL2-family regulator whose activity is strongly controlled by phosphorylation. When active, BAD can engage anti-apoptotic BCL2-family proteins and shift mitochondrial signalling toward intrinsic apoptosis. This page groups ABMIUM catalogue reagents for BAD signalling and cell-death research.

Biological function: BAD is regulated by phosphorylation at multiple serine residues. Survival signalling can promote phosphorylated BAD association with 14-3-3 proteins, limiting its pro-apoptotic activity. Dephosphorylated BAD can bind anti-apoptotic BCL2-family proteins such as BCL-XL and BCL2, favouring activation of the mitochondrial apoptosis programme.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
572
UniProtKB
Q92934
Elabscience
£407.00£325.60