Mitochondrial Membrane Potential Assay Kit (with JC-1)

The effect of this kit to detect apoptosis induced by camptothecin in Jurkat cells is shown: normal cells (left) have a small amount of apoptosis, which is manifested as a small amount of mitochondrial membrane potential collapse cells; induced apoptotic cells (middle, 2.5 μM camptotheca Alkaline-treated Jurkat cells for 24 h) had a large number of mitochondrial membrane potential collapse cells; CCCP-treated cells (right, positive control) almost all cells had mitochondrial membrane potential collapse.

The effect of this kit to detect apoptosis induced by camptothecin in Jurkat cells is shown: normal cells (left) have a small amount of apoptosis, which is manifested as a small amount of mitochondrial membrane potential collapse cells; induced apoptotic cells (middle, 2.5 μM camptotheca Alkaline-treated Jurkat cells for 24 h) had a large number of mitochondrial membrane potential collapse cells; CCCP-treated cells (right, positive control) almost all cells had mitochondrial membrane potential collapse.
Manufactured by:Elabscience
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Mitochondrial Membrane Potential Assay Kit (with JC-1)

Mitochondrial Membrane Potential Assay Kit (with JC-1) with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM10769-1
Product type
Assay Kits

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Mitochondrial Membrane Potential Assay Kit (with JC-1) from Elabscience is intended for research workflows. Review the listed specifications and supporting documents to confirm compatibility with the intended method.

Key facts & specifications
Incubation temperature
This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Assay type
Assay Kits
Detection method
Fluorometric method,Flow cytometry
Technical note
The decrease of mitochondrial membrane potential is a marker event in the early stage of apoptosis. It occurs before the appearance of nuclear apoptotic features (chromatin condensation and DNA fragmentation). Once the mitochondrial membrane potential collapses, apoptosis is irreversible. JC-1 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential ?Ψm. In normal cells, the mitochondrial membrane potential is high, JC-1 aggregates in the mitochondrial matrix to form a polymer, which can produce red fluorescence. In the early stage of apoptosis, the mitochondrial membrane potential decreases, JC-1 can’t accumulate in the mitochondrial matrix. When JC-1 is a monomer, it can produce green fluorescence. The decrease of cell membrane potential can be detected by the transition of JC-1 from red fluorescence to green fluorescence, and the transition of JC-1 fluorescence color can be used as an early detection indicator of cell apoptosis. The relative ratio of red and green fluorescence is commonly used to measure the ratio of mitochondrial depolarization. The maximum excitation wavelength of JC-1 monomer is 514 nm and the maximum emission wavelength is 529 nm; the maximum excitation wavelength of JC-1 polymer is 585 nm and the maximum emission wavelength is 590 nm.
Identifiers & database references
Supplier SKU / catalogue number
E-CK-A301_100Assays
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Elabscience
£275.00£220.00