Manufactured by:ELK Biotechnology
RUO

C8 α rabbit pAb

C8 α rabbit pAb from ELK Biotechnology

SKU:
ABM109805
Product type
Primary Antibody
TargetC8 α
ApplicationsWB, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemBlood,Liver,Plasma,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

C8 α rabbit pAb is a research-use antibody from ELK Biotechnology for studying C8 α in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

C8 is a component of the complement system and contains three polypeptides, alpha, beta and gamma. This gene encodes the alpha subunit of C8. C8 participates in the formation of the membrane attack complex (MAC). The MAC assembles on bacterial membranes to form a pore, permitting disruption of bacterial membrane organization. Mutations in this gene cause complement C8 alpha-gamma deficiency. [provided by RefSeq, Nov 2008],

Additional antibody information

Specificity C8 α Polyclonal Antibody detects endogenous levels of C8 α
Validation evidence captions Western Blot analysis of 1,mouse-kidney 2,mouse-heart cells using primary antibody diluted at 1:500(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)
Key facts & specifications
Research areas
>>Complement and coagulation cascades, >>Prion disease, >>Amoebiasis, >>Coronavirus disease - COVID-19, >>Systemic lupus erythematosus
Also known as
Complement component C8 alpha chain (Complement component 8 subunit alpha)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Immunogen
Synthesized peptide derived from human C8 α. at AA range: 201-250
Observed band
60kD
Cellular localization
Secreted. Cell membrane; Multi-pass membrane protein. Secreted as soluble protein. Inserts into the cell membrane of target cells.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 201-250
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES8864
UniProt entry
P07357
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00