Manufactured by:ELK Biotechnology
RUO

AMBRA1 rabbit pAb

AMBRA1 rabbit pAb from ELK Biotechnology

SKU:
ABM109680
Product type
Primary Antibody
TargetAMBRA1
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse
Expression systemBrain,Testis,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

AMBRA1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying AMBRA1 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation.,similarity:Contains 3 WD repeats.,subunit:Interacts with BECN1. Probably forms a complex with BECN1 and PIK3C3.,

Additional antibody information

Specificity AMBRA1 Polyclonal Antibody detects endogenous levels of AMBRA1
Validation evidence captions Western Blot analysis of 293T 3T3 cells using AMBRA1 Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Key facts & specifications
Research areas
>>Mitophagy - animal, >>Autophagy - animal, >>Alzheimer disease, >>Amyotrophic lateral sclerosis, >>Huntington disease, >>Spinocerebellar ataxia, >>Pathways of neurodegeneration - multiple diseases
Also known as
autophagy/beclin-1 regulator 1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000, ELISA 1:10000-20000
Immunogen
Synthetic Peptide of AMBRA1
Observed band
140kD
Cellular localization
Endoplasmic reticulum . Cytoplasm, cytoskeleton . Cytoplasmic vesicle, autophagosome . Mitochondrion . Cytoplasm, cytosol . Nucleus . Cell junction, focal adhesion . Localizes to the cytoskeleton in absence of autophagy induction (PubMed:20921139). Upon autophagy induction, AMBRA1 relocalizes to the endoplasmic reticulum to enable autophagosome nucleation (PubMed:20921139). Partially localizes at mitochondria in normal conditions (PubMed:21358617). Localizes also to discrete punctae along the ciliary axoneme (By similarity). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000, ELISA 1:10000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES8480
UniProt entry
Q9C0C7
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00