IFN-α/βRα rabbit pAb

IFN-α/βRα rabbit pAb

IFN-α/βRα rabbit pAb
Manufactured by:ELK Biotechnology
RUO

IFN-α/βRα rabbit pAb

IFN-α/βRα rabbit pAb from ELK Biotechnology

SKU:
ABM108753
Product type
Primary Antibody
TargetIFN-α/βRα
ApplicationsIHC, IF, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemIFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

IFN-α/βRα rabbit pAb is a research-use antibody from ELK Biotechnology for studying IFN-α/βRα in IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity IFN-α/βRα Polyclonal Antibody detects endogenous levels of IFN-α/βRα protein.
Validation evidence captions Immunofluorescence analysis of HepG2 cells, using Interferon-alpha/beta Receptor alpha chain Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Interferon-alpha/beta Receptor alpha chain Antibody. The picture on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>Cytokine-cytokine receptor interaction, >>PI3K-Akt signaling pathway, >>Necroptosis, >>Osteoclast differentiation, >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>JAK-STAT signaling pathway, >>Natural killer cell mediated cytotoxicity, >>Hepatitis C, >>Hepatitis B, >>Measles, >>Influenza A, >>Human papillomavirus infection, >>Kaposi sarcoma-associated herpesvirus infection, >>Herpes simplex virus 1 infection, >>Epstein-Barr virus infection, >>Coronavirus disease - COVID-19, >>Pathways in cancer
Also known as
IFNAR1; IFNAR; Interferon alpha/beta receptor 1; IFN-R-1; IFN-alpha/beta receptor 1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; CRF2-1; Type I interferon receptor 1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000 ,Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human Interferon-alpha/beta Receptor alpha chain. AA range:436-485
Cellular localization
[Isoform 1]: Cell membrane ; Single-pass type I membrane protein . Late endosome . Lysosome . Interferon binding triggers internalization of the receptor from the cell membrane into endosomes and then into lysosomes. .
Assay type
Antibody validation/application
Source organism
Rabbit
Molecular weight
64kD
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ,Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 436-485
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES5837
UniProt entry
P17181
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00