Manufactured by:ELK Biotechnology
RUO

Gab 1 (phospho Tyr659) rabbit pAb

Gab 1 (phospho Tyr659) rabbit pAb from ELK Biotechnology

SKU:
ABM108566
Product type
Primary Antibody
TargetGab 1
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemTestis,Whole embryo,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Gab 1 (phospho Tyr659) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Gab 1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

GRB2 associated binding protein 1(GAB1) Homo sapiens The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008],

Additional antibody information

Specificity Phospho-Gab 1 (Y659) Polyclonal Antibody detects endogenous levels of Gab 1 protein only when phosphorylated at Y659.
Validation evidence captions Western Blot analysis of 3T3 cells using Phospho-Gab 1 (Y659) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using GAB1 (Phospho-Tyr659) Antibody | Immunofluorescence analysis of HepG2 cell, using GAB1 (Phospho-Tyr659) Antibody. The lane on the right is blocked with the GAB1 (Phospho-Tyr659) peptide. | Immunohistochemistry analysis of paraffin-embedded human breast cancer, using GAB1 (Phospho-Tyr659) Antibody. The picture on the right is blocked with the GAB1 (Phospho-Tyr659) peptide.
Key facts & specifications
Research areas
>>EGFR tyrosine kinase inhibitor resistance, >>ErbB signaling pathway, >>Ras signaling pathway, >>Phospholipase D signaling pathway, >>Neurotrophin signaling pathway, >>Bacterial invasion of epithelial cells, >>Proteoglycans in cancer, >>Renal cell carcinoma, >>Hepatocellular carcinoma, >>Gastric cancer
Also known as
GAB1; GRB2-associated-binding protein 1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human GAB1 around the phosphorylation site of Tyr659. AA range:644-674
Observed band
76kD
Cellular localization
cytosol,
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 644-674
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES5422
UniProt entry
Q13480
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00