Manufactured by:ELK Biotechnology
RUO

Dynamin I (phospho Ser778) rabbit pAb

Dynamin I (phospho Ser778) rabbit pAb from ELK Biotechnology

SKU:
ABM108397
Product type
Primary Antibody
TargetDynamin I
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemBrain,Platelet,PNS,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Dynamin I (phospho Ser778) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Dynamin I in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

dynamin 1(DNM1) Homo sapiens This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Phospho-Dynamin I (S778) Polyclonal Antibody detects endogenous levels of Dynamin I protein only when phosphorylated at S778.
Validation evidence captions Western Blot analysis of Mouse-Brain cells using Phospho-Dynamin I (S778) Polyclonal Antibody | Western blot analysis of DYN1 (Phospho-Ser778) Antibody. The lane on the right is blocked with the DYN1 (Phospho-Ser778) peptide.
Key facts & specifications
Research areas
>>Phospholipase D signaling pathway, >>Endocytosis, >>Synaptic vesicle cycle, >>Endocrine and other factor-regulated calcium reabsorption, >>Bacterial invasion of epithelial cells
Also known as
DNM1; DNM; Dynamin-1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human DYN1 around the phosphorylation site of Ser778. AA range:751-800
Observed band
100kD
Cellular localization
Cytoplasm . Cytoplasm, cytoskeleton . Microtubule-associated.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 751-800
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES5009
UniProt entry
Q05193
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00