Manufactured by:ELK Biotechnology
RUO

TNF-β rabbit pAb

TNF-β rabbit pAb from ELK Biotechnology

SKU:
ABM107590
Product type
Primary Antibody
TargetTNFB
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat, Monkey
Expression systemLymph,Peripheral blood leukocyte,Peripheral blood mononuclear cells,Peripheral blood mononu
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

TNF-β rabbit pAb is a research-use antibody from ELK Biotechnology for studying TNFB in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012],

Additional antibody information

Specificity TNF-β Polyclonal Antibody detects endogenous levels of TNF-β protein.
Validation evidence captions Western Blot analysis of various cells using TNF-β Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from COS-7cell, using TNF-β antibodys.
Key facts & specifications
Research areas
>>Cytokine-cytokine receptor interaction, >>Viral protein interaction with cytokine and cytokine receptor, >>NF-kappa B signaling pathway, >>TNF signaling pathway, >>Type I diabetes mellitus, >>Human T-cell leukemia virus 1 infection, >>Herpes simplex virus 1 infection
Also known as
LTA; TNFB; TNFSF1; Lymphotoxin-alpha; LT-alpha; TNF-beta; Tumor necrosis factor ligand superfamily member 1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human TNF-beta. AA range:17-66
Observed band
22kD
Cellular localization
Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 17-66
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Monkey
Identifiers & database references
Supplier SKU / catalogue number
ES3624
UniProt entry
P01374
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00