Manufactured by:ELK Biotechnology
RUO

Pdcd-4 rabbit pAb

Pdcd-4 rabbit pAb from ELK Biotechnology

SKU:
ABM107413
Product type
Primary Antibody
TargetPDCD4
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Pdcd-4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying PDCD4 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010],

Additional antibody information

Specificity Pdcd-4 Polyclonal Antibody detects endogenous levels of Pdcd-4 protein.
Validation evidence captions Western Blot analysis of various cells using Pdcd-4 Polyclonal Antibody | Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Immunofluorescence analysis of HeLa cells, using PDCD4 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from 293 cells, using PDCD4 Antibody. The lane on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>Proteoglycans in cancer, >>MicroRNAs in cancer
Also known as
PDCD4; H731; Programmed cell death protein 4; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human PDCD4. AA range:420-469
Observed band
51kD
Cellular localization
Nucleus . Cytoplasm . Shuttles between the nucleus and cytoplasm (By similarity). Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457 (PubMed:16357133). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 420-469
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES3161
UniProt entry
Q53EL6
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

PDCD4programmed cell death 4

View target guide

PDCD4 identifies programmed cell death 4 in Homo sapiens (Human). This target record connects NCBI Gene 27250, UniProtKB Q53EL6 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
27250
UniProtKB
Q53EL6
ELK Biotechnology
£220.00