Manufactured by:ELK Biotechnology
RUO

GCSc-γ rabbit pAb

GCSc-γ rabbit pAb from ELK Biotechnology

SKU:
ABM107084
Product type
Primary Antibody
TargetGCSc-γ
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemForeskin fibroblast,Hippocampus,Liver,Testis,Thalamus,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

GCSc-γ rabbit pAb is a research-use antibody from ELK Biotechnology for studying GCSc-γ in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010],

Additional antibody information

Specificity GCSc-γ Polyclonal Antibody detects endogenous levels of GCSc-γ protein.
Validation evidence captions Western Blot analysis of various cells using GCSc-γ Polyclonal Antibody | Western blot analysis of lysate from A549 cells, using GCSc-γ antibody.
Key facts & specifications
Research areas
>>Cysteine and methionine metabolism, >>Glutathione metabolism, >>Metabolic pathways, >>Biosynthesis of cofactors, >>Ferroptosis
Also known as
GCLC; GLCL; GLCLC; Glutamate--cysteine ligase catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human GCSc-gamma. AA range:266-315
Observed band
73kD
Cellular localization
cytosol,integral component of membrane,glutamate-cysteine ligase complex,
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 266-315
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES2414
UniProt entry
P48506
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00