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Manufactured by:ELK Biotechnology
RUO

Actin β rabbit pAb

Actin β rabbit pAb from ELK Biotechnology

SKU:
ABM105602
Product type
Primary Antibody
TargetActin β
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat, Chicken, Globefish, Bovine, Hamster, Pig, Ovine, Cat, Dog
Expression systemB-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Actin β rabbit pAb is a research-use antibody from ELK Biotechnology for studying Actin β in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Chicken, Globefish, Bovine, Hamster, Pig, Ovine, Cat, Dog. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Actin β Polyclonal Antibody detects endogenous levels of Actin β protein.
Key facts & specifications
Research areas
>>Rap1 signaling pathway, >>Phagosome, >>Apoptosis, >>Hippo signaling pathway, >>Focal adhesion, >>Adherens junction, >>Tight junction, >>Platelet activation, >>Neutrophil extracellular trap formation, >>Leukocyte transendothelial migration, >>Thermogenesis, >>Regulation of actin cytoskeleton, >>Thyroid hormone signaling pathway, >>Oxytocin signaling pathway, >>Gastric acid secretion, >>Amyotrophic lateral sclerosis, >>Bacterial invasion of epithelial cells, >>Vibrio cholerae infection, >>Pathogenic Escherichia coli infection, >>Shigellosis, >>Salmonella infection, >>Yersinia infection, >>Influenza A, >>Proteoglycans in cancer, >>Hepatocellular carcinoma, >>Hypertrophic cardiomyopathy, >>Arrhythmogenic right ventricular cardiomyopathy, >>Dilated cardiomyopathy, >>Viral myocarditis, >>Fluid shear stress and atherosclerosis
Also known as
ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β;β-Actin;Beta actin
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
IF: 1:50-200 Western Blot: 1/2000 - 1/10000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
Synthesized peptide derived from the N-terminal region of human Actin β. AA range: 1-80
Observed band
42kD
Cellular localization
Cytoplasm, cytoskeleton . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
IF: 1:50-200 Western Blot: 1/2000 - 1/10000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 1-80
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Chicken
Globefish
Bovine
Hamster
Pig
Ovine
Cat
Dog
Identifiers & database references
Supplier SKU / catalogue number
ES1592
UniProt entry
P60709
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00