Manufactured by:ELK Biotechnology
RUO

FoxO4 (phospho Ser197) rabbit pAb

FoxO4 (phospho Ser197) rabbit pAb from ELK Biotechnology

SKU:
ABM104394
Product type
Primary Antibody
TargetFoxO4
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse
Expression systemHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

FoxO4 (phospho Ser197) rabbit pAb is a research-use antibody from ELK Biotechnology for studying FoxO4 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010],

Additional antibody information

Specificity Phospho-FoxO4 (S197) Polyclonal Antibody detects endogenous levels of FoxO4 protein only when phosphorylated at S197.
Validation evidence captions Western Blot analysis of various cells using Phospho-FoxO4 (S197) Polyclonal Antibody | Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using AFX (Phospho-Ser197) Antibody | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AFX (Phospho-Ser197) Antibody. The picture on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Ras signaling pathway, >>FoxO signaling pathway, >>Shigellosis
Also known as
FOXO4; AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human AFX around the phosphorylation site of Ser197. AA range:164-213
Observed band
70kD
Cellular localization
Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 164-213
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES1316
UniProt entry
P98177
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

FOXO4forkhead box O4

View target guide

FOXO4 identifies forkhead box O4 in Homo sapiens (Human). This target record connects NCBI Gene 4303, UniProtKB P98177 and related ABMIUM catalogue products.

Organism
Homo sapiens (Human)
NCBI Gene
4303
UniProtKB
P98177
ELK Biotechnology
£220.00