Manufactured by:ELK Biotechnology
RUO

Recombinant Human EpCAM (C-Fc)

Recombinant Human EpCAM (C-Fc) from ELK Biotechnology

SKU:
ABM100905
Product type
Recombinant Proteins
TargetEpCAM
ApplicationsProtein research
Purity>95% by SDS-PAGE

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Recombinant Human EpCAM (C-Fc) is a research-use recombinant protein from ELK Biotechnology preparation representing EpCAM supplied as Lyophilized. Review the expression, purity, formulation and storage specifications below before selecting it for an assay or functional workflow.

Description

Recombinant Human Epithelial Cell Adhesion Molecule is produced by our Mammalian expression system and the target gene encoding Gln24-Lys265 is expressed with a Fc tag at the C-terminus.

Background

Epithelial Cell Adhesion Molecule (EpCAM) is a signal type I transmembrane glycoprotein that belongs to the EPCAM family. EpCAM is composed of an extracellular domain with one thyroglobulin type-1 domain, a transmembrane domain and a cytoplasmic domain. EpCAM is found on the surface of adenocarcinoma, but not on mesodermal or neural cell membranes. The EpCAM molecule has been shown to function as a homophilic Ca2+ independent adhesion molecule. It may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium as an immunological barrier providing the first line of defense against infection. Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) and diarrhea type 5 (DIAR5). EpCAM plays a role in embryonic stem cells proliferation and differentiation; it up-regulates the expression of FABP5, MYC and Cyclin A and Cyclin E. It is highly and selectively expressed by undifferentiated embryonic stem cells.

Handling information

Reconstitution Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100µg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Delivery condition The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Key facts & specifications
Research areas
Molecular Biology
Also known as
Epithelial Cell Adhesion Molecule; Ep-CAM; Adenocarcinoma-Associated Antigen; Cell Surface Glycoprotein Trop-1; Epithelial Cell Surface Antigen; Epithelial Glycoprotein 314; EGP314; Major Gastrointestinal Tumor-Associated Protein GA733-2; Tumor-Associated Calcium Signal Transducer 1; CD326; EPCAM; GA733-2; TROP1
Assay type
Recombinant protein
Molecular weight
54.5 KDa
Form / buffer
Lyophilized
Endotoxin level
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Quantity per pack
50ug
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpecificationPurityEndotoxinBioactivity
Product QC
Identifiers & database references
Supplier SKU / catalogue number
EPT181
UniProt entry
AAH14785.1
Storage, stability & shipping
Storage conditions
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Long-term storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

EPCAMepithelial cell adhesion molecule

View target guide

EPCAM identifies epithelial cell adhesion molecule in Homo sapiens (Human). This target record connects NCBI Gene 4072, UniProtKB P16422 and related ABMIUM catalogue products.

Biological function: EPCAM is an epithelial cell-surface protein implicated in epithelial barrier interactions and regulation of cell proliferation and differentiation, including roles described in intestinal epithelium and embryonic stem-cell contexts.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
4072
UniProtKB
P16422
ELK Biotechnology
£301.00