Human ACTN3(Actinin Alpha 3) Microsample ELISA Kit from ELK Biotechnology
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Human ACTN3(Actinin Alpha 3) Microsample ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring ACTN3 in Tissue homogenates, Cell lysates, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACTN3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACTN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACTN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACTN3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein.
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Sample compatibility evidence using the same validation key.
| Sample type | Tissue homogenates | Cell lysates | Other biological fluids |
|---|---|---|---|
| Sample compatibility |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
Actinin alpha 3 (ACTN3), encoding alpha-actinin-3, is a skeletal-muscle actin-binding protein concentrated in fast type 2 fibres. This record connects NCBI Gene 89, reviewed UniProtKB Q08043 and related ABMIUM catalogue products.
Biological function: Alpha-actinin-3, encoded by ACTN3 (actinin alpha 3), is an F-actin cross-linking and bundling protein that contributes to the sarcomeric Z line. It is primarily associated with skeletal muscle, with reviewed UniProt reporting protein expression restricted to fast type 2 skeletal-muscle fibres.
