Manufactured by:ELK Biotechnology
RUO

Rat ALDOA(Aldolase A, Fructose Bisphosphate) ELISA Kit

Rat ALDOA(Aldolase A, Fructose Bisphosphate) ELISA Kit from ELK Biotechnology

SKU:
ABM63041
Product type
ELISA Kits
TargetALDOA
ApplicationsELISA
Species / reactivityRat
Sample typeSerum, Plasma, Tissue homogenates, Other biological fluids

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Rat ALDOA(Aldolase A, Fructose Bisphosphate) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring ALDOA in Serum, Plasma, Tissue homogenates, Other biological fluids samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ALDOA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ALDOA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ALDOA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ALDOA in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include FBA; ALDO-A; Fructose 1,6 Bisphosphate Aldolase; Lung cancer antigen NY-LU-1; Muscle-type aldolase.

Key facts & specifications
Research areas
Enzyme & Kinase, Metabolic pathway, Hepatology
Also known as
FBA; ALDO-A; Fructose 1,6 Bisphosphate Aldolase; Lung cancer antigen NY-LU-1; Muscle-type aldolase
Assay type
Sandwich
Detection method
Colorimetric (TMB/HRP)
Sensitivity
0.063 ng/mL
Detection range
0.16-10 ng/mL
Plate format
96-well pre-coated
Form / buffer
Pre-coated microplate kit
Quantity per pack
48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typeSerumPlasmaTissue homogenatesOther biological fluids
Sample compatibility
Identifiers & database references
Supplier SKU / catalogue number
ELK3352
UniProt entry
P05065
Storage, stability & shipping
Storage conditions
Store according to the conditions stated in the product manual.
Long-term storage
Store according to the conditions stated in the product manual.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ALDOAaldolase, fructose-bisphosphate A

View target guide

ALDOA encodes aldolase, fructose-bisphosphate A; reviewed UniProt names the protein fructose-bisphosphate aldolase A. Also known as muscle-type aldolase, it is a class I glycolytic enzyme that reversibly cleaves fructose 1,6-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.

Biological function: Fructose-bisphosphate aldolase A catalyses a central reversible step in glycolysis and gluconeogenesis, interconverting fructose 1,6-bisphosphate with two triose phosphates. Reviewed UniProt also supports non-catalytic and scaffolding roles linked to metabolic responses to glucose availability.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
226
UniProtKB
P04075
ELK Biotechnology
£284.00