Manufactured by:Boster Bio
ABMIUM Verified™RUO

Anti-TRIM25 Monoclonal Antibody

Primary Antibody supplied by Boster Bio

SKU:
ABM29481
Product type
Primary Antibody
TargetE3 ubiquitin/ISG15 ligase TRIM25
ApplicationsFlow Cytometry, IF/ICC, IHC, IP, WB
Species / reactivityHuman, Mouse, Rat

Manufacturer provenance

Manufactured by:
Boster Bio
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Anti-TRIM25 Monoclonal Antibody is a research-use antibody from Boster Bio for studying E3 ubiquitin/ISG15 ligase TRIM25 in Flow Cytometry, IF/ICC, IHC, IP, WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-TRIM25 Monoclonal Antibody catalog # M03232. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference M03232
Product category Primary Antibodies, Rabbit Monoclonal Antibodies
Reactive species Human, Mouse, Rat
Tested applications Flow Cytometry, IF/ICC, IHC, IP, WB
Available conjugations Unconjugated
Gene name TRIM25
Protein name E3 ubiquitin/ISG15 ligase TRIM25
Immunogen A synthesized peptide derived from human TRIM25 Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection.
Host Rabbit
Clonality Monoclonal
Isotype Rabbit IgG
Purification Affinity-chromatography
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
UniProt Q14258
NCBI Gene ID 7706

Available variants

Conjugation Size
Unconjugated 100 μl/vial
Key facts & specifications
Also known as
EFP; RING finger protein 147; RNF147; TRIM25; tripartite motif containing 25; Z147; Zinc finger protein 147; ZNF147
Host species
Rabbit
Clonality
Monoclonal
Clone number
Clone: ADFG-20
Isotype
Rabbit IgG
Dilution range
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:60
Immunogen
A synthesized peptide derived from human TRIM25 Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection.
Observed band
71 kDa
Molecular weight
23743 MW
Concentration
0.5mg/ml
Purification
Affinity-chromatography
Form / buffer
Liquid
Sequence fragment
A synthesized peptide derived from human TRIM25 Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
M03232
Gene symbol
TRIM25
Gene ID
7706
UniProt accession
Q14258
Storage, stability & shipping
Shelf life
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage buffer
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Compliance & documents
CE marked
No
Datasheet
Open datasheet
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Boster Bio and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Boster Bio
£328.00