Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit

Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit - ABMIUM

Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit - ABMIUM
Manufactured by:Elabscience
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Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit

Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM10969-1
Product type
Assay Kits
TargetNOS3

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit from Elabscience is intended for research workflows involving NOS3. Review the listed specifications and supporting documents to confirm compatibility with the intended method.

Key facts & specifications
Research areas
Cancer, Cell Biology, Cardiovascular, Metabolism, Neuroscience
Also known as
ECNOS,NOS III,E-NOS,eNOS,NOS3,
Cross-reactivity
This kit recognizes Human NOS3/eNOS in samples.No significant cross-reactivity or interference between Human NOS3/eNOS and analogues was observed
Incubation temperature
2-8℃/-20℃
Assay type
CLIA Kits
Detection method
Chemiluminescence
Plate format
48-well microplate plates pre-coated with anti-NOS3 capture antibody
Technical note
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NOS3/eNOS. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human NOS3/eNOS. You can calculate the concentration of Human NOS3/eNOS in the samples by comparing the RLU value of the samples to the standard curve.
Identifiers & database references
Supplier SKU / catalogue number
E-CL-H0543_48T
Gene symbol
NOS3
Gene ID
4846
UniProt accession
P29474
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

NOS3nitric oxide synthase 3

View target guide

NOS3 identifies nitric oxide synthase 3 in Homo sapiens (Human). This target record connects NCBI Gene 4846, UniProtKB P29474 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
4846
UniProtKB
P29474
Browse all NOS3 productsRead target context & evidence
Elabscience
£407.00£325.60