Manufactured by:Elabscience
Save 20%ABMIUM Verified™RUO

Human APAF1 (Apoptosis Protease Activating Factor 1) CLIA Kit

Human APAF1 (Apoptosis Protease Activating Factor 1) CLIA Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM10931-1
Product type
Assay Kits
TargetAPAF1

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Human APAF1 (Apoptosis Protease Activating Factor 1) CLIA Kit from Elabscience is intended for research workflows involving APAF1. Review the listed specifications and supporting documents to confirm compatibility with the intended method.

Key facts & specifications
Cross-reactivity
This kit recognizes Human APAF1 in samples.No significant cross-reactivity or interference between Human APAF1 and analogues was observed
Incubation temperature
2-8℃/-20℃
Assay type
CLIA Kits
Detection method
Chemiluminescence
Plate format
48-well microplate plates pre-coated with anti-APAF1 capture antibody
Technical note
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human APAF1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human APAF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human APAF1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human APAF1. You can calculate the concentration of Human APAF1 in the samples by comparing the RLU value of the samples to the standard curve.
Identifiers & database references
Supplier SKU / catalogue number
E-CL-H0393_48T
Gene symbol
APAF1
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

APAF1apoptotic peptidase activating factor 1

View target guide

APAF1 (apoptotic peptidase activating factor 1; Apaf-1) is a core scaffold of the intrinsic apoptosis pathway. Following mitochondrial cytochrome c release, APAF1 assembles the apoptosome and recruits procaspase-9, linking mitochondrial stress to downstream caspase activation.

Biological function: APAF1 binds cytochrome c and ATP or dATP, then oligomerizes into the apoptosome. Through its CARD domain, the complex recruits and activates procaspase-9, which initiates downstream executioner-caspase signalling including caspase-3 activation.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
317
UniProtKB
O14727
Elabscience
£407.00£325.60