β-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit

β-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit - ABMIUM

β-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit - ABMIUM
Manufactured by:Elabscience
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β-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit

β-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM10328-1
Product type
Assay Kits
TargetBACE 1

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

β-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit from Elabscience is intended for research workflows involving BACE 1. Review the listed specifications and supporting documents to confirm compatibility with the intended method.

Key facts & specifications
Research areas
Amino Acids and Proteins
Also known as
BACE 1
Incubation temperature
This product can be stored at -20°C for 12 months with shading light.
Assay type
Assay Kits
Detection method
Fluorometric method
Technical note
β-Secretase 1 is an aspartic protease, the full name is β-site amyloid precursor protein cleavage enzyme 1(BACE 1). BACE 1 cleaves amyloid precursor protein (APP) to produce amyloid-β peptide (Aβ) of 40 or 42 amino acids. The deposition of Aβ in plaques and vessel walls in the brains of patients with Alzheimer's disease is widely believed to be the major contributor to the disease.
The detection principle of this kit is fluorescence resonance energy transfer (FRET) method. A fluorescence donor group and a quenching group are divided at both ends of the substrate. The fluorescence energy is transferred from the fluorescence donor to the quenching group, resulting in the attenuation of the fluorescence intensity of the fluorescence donor molecule itself. When the substrate is cleaved by BACE 1, the quenching group of the substrate and the fluorescent donor are separated and the fluorescent donor is no longer quenched, the fluorescence of the fluorescent donor can be detected, so that the enzymatic activity of BACE 1 can be calculated. The fluorescence produced by this kit had a maximum excitation wavelength of 325 nm and a maximum absorption wavelength of 393 nm.
Identifiers & database references
Supplier SKU / catalogue number
E-BC-F076_48T
Gene symbol
BACE 1
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Elabscience
£261.00£208.80