Manufactured by:Boster Bio
ABMIUM Verified™RUO

Mouse TNF Alpha High Sensitivity ELISA Kit PicoKine®

ELISA Kit supplied by Boster Bio

SKU:
ABM34605
Product type
ELISA Kits
TargetTNF
ApplicationsELISA
Species / reactivityMouse
Sample typeCell culture supernatant, Cell lysates, serum and plasma (heparin, EDTA).
PurityNatural and recombinant Mouse TNF alpha

Manufacturer provenance

Manufactured by:
Boster Bio
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Mouse TNF Alpha High Sensitivity ELISA Kit PicoKine® is a research-use ELISA kit from Boster Bio for measuring Tumor necrosis factor in Cell culture supernatant, Cell lysates, serum and plasma (heparin, EDTA). samples from Mouse. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Mouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 0.006pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Tumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.

Product information

Key facts & specifications
Also known as
Cachectin; DIF; TNF; TNF a; TNF alpha; TNFA; TNF-a; TNF-alpha; TNFSF2; TNFα; TNF-α; Tumor necrosis factor; tnfa; tnfα
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: E.coli; Immunogen sequence: L80-L235
Sensitivity
<0.006 pg/ml
Detection range
1.56-100pg/ml
Sequence fragment
Expression system for standard: E.coli; Immunogen sequence: L80-L235
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typeSample CompatibilityAssay RangeSensitivitySpecificity
cell culture supernatants
cell lysates
serum and plasma (heparin
EDTA).
Identifiers & database references
Supplier SKU / catalogue number
HSEK0527
Gene symbol
TNF
Gene ID
21926
UniProt accession
P06804
Storage, stability & shipping
Shelf life
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Storage conditions
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Long-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Compliance & documents
CE marked
No
Datasheet
Open datasheet
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Boster Bio and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

TNFTumor necrosis factor

View target guide

TNF encodes a multifunctional pro-inflammatory cytokine in the tumour necrosis factor superfamily. This target hub connects accepted names and identifiers with ABMIUM antibodies, ELISA kits and related assay products.

Biological function: TNF is produced as a membrane-associated cytokine and can be released in soluble form. Signalling through TNFRSF1A (TNFR1) and TNFRSF1B (TNFR2) contributes to regulation of inflammation, immune responses, cell survival, differentiation and cell death.

Organism
Homo sapiens (Human)
NCBI Gene
7124
UniProtKB
P01375
Boster Bio
£460.00