Manufactured by:ELK Biotechnology
RUO

RXRA rabbit pAb

RXRA rabbit pAb from ELK Biotechnology

SKU:
ABM109872
Product type
Primary Antibody
TargetRXRA
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat, Bovine
Expression systemExpressed in lung fibroblasts (at protein level) (PubMed:30216632). Expressed in monocytes (PubMed:26463675). Highly expressed in liver, also found in kidney and brain (PubMed:24275569, PubMed:2159111, PubMed:14702039).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

RXRA rabbit pAb is a research-use antibody from ELK Biotechnology for studying RXRA in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat, Bovine. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

retinoid X receptor alpha(RXRA) Homo sapiens Retinoid X receptors (RXRs) and retinoic acid receptors (RARs) are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors function as transcription factors by binding as homodimers or heterodimers to specific sequences in the promoters of target genes. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014],

Additional antibody information

Specificity RXRA Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
>>PPAR signaling pathway, >>PI3K-Akt signaling pathway, >>Th17 cell differentiation, >>Thyroid hormone signaling pathway, >>Adipocytokine signaling pathway, >>Parathyroid hormone synthesis, secretion and action, >>Non-alcoholic fatty liver disease, >>Bile secretion, >>Hepatitis C, >>Pathways in cancer, >>Transcriptional misregulation in cancer, >>Chemical carcinogenesis - receptor activation, >>Thyroid cancer, >>Small cell lung cancer, >>Non-small cell lung cancer, >>Gastric cancer, >>Lipid and atherosclerosis
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Immunogen
Synthesized peptide derived from human protein . at AA range: 200-280
Observed band
50kD
Cellular localization
Nucleus . Cytoplasm . Mitochondrion . Localization to the nucleus is enhanced by vitamin D3 (PubMed:15509776). Nuclear localization may be enhanced by the interaction with heterodimerization partner VDR (PubMed:12145331). Translocation to the mitochondrion upon interaction with NR4A1 (PubMed:17761950, PubMed:15509776). Increased nuclear localization upon pulsatile shear stress (PubMed:28167758). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 200-280
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Bovine
Identifiers & database references
Supplier SKU / catalogue number
ES8964
UniProt entry
P19793
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

RXRAretinoid X receptor alpha

View target guide

RXRA identifies retinoid X receptor alpha in Homo sapiens (Human). This target record connects NCBI Gene 6256, UniProtKB P19793 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6256
UniProtKB
P19793
ELK Biotechnology
£220.00