Manufactured by:ELK Biotechnology
RUO

Vimentin (Phospho-Tyr38) rabbit pAb

Vimentin (Phospho-Tyr38) rabbit pAb from ELK Biotechnology

SKU:
ABM109667
Product type
Primary Antibody
TargetVimentin
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Vimentin (Phospho-Tyr38) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Vimentin in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009],

Additional antibody information

Specificity This antibody detects endogenous levels of Vimentin at Human:Y38;Mouse:Y38;Rat:Y38, It doesn't reacte with total protein.
Validation evidence captions Western Blot analysis of HELA cells using Antibody diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Vimentin (Phospho-Ser38) Antibody | Western blot analysis of lysates from 293 cells treated with paclitaxel, using p-Vimentin (Phospho-Ser38) antibody.
Key facts & specifications
Research areas
>>Epstein-Barr virus infection, >>MicroRNAs in cancer
Also known as
VIM
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB: 1:500-10000 ELISA: 1:10000
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human Vimentin (Phospho-Tyr38)
Observed band
51kD
Cellular localization
Cytoplasm . Cytoplasm, cytoskeleton . Nucleus matrix . Cell membrane .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB: 1:500-10000 ELISA: 1:10000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES8459
UniProt entry
P08670
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00