Manufactured by:ELK Biotechnology
RUO

IL-13Rα1 (phospho Tyr405) rabbit pAb

IL-13Rα1 (phospho Tyr405) rabbit pAb from ELK Biotechnology

SKU:
ABM108778
Product type
Primary Antibody
TargetIL-13Rα1
ApplicationsWB, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemUbiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

IL-13Rα1 (phospho Tyr405) rabbit pAb is a research-use antibody from ELK Biotechnology for studying IL-13Rα1 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Phospho-IL-13Rα1 (Y405) Polyclonal Antibody detects endogenous levels of IL-13Rα1 protein only when phosphorylated at Y405.
Validation evidence captions Western Blot analysis of 3T3 cells using Phospho-IL-13Rα1 (Y405) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IL-13R alpha1 (Phospho-Tyr405) Antibody | Immunofluorescence analysis of HepG2 cells, using IL-13R alpha1 (Phospho-Tyr405) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HUVEC cells treated with serum 20% 15', using IL-13R alpha1 (Phospho-Tyr405) Antibody. The lane on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Cytokine-cytokine receptor interaction, >>JAK-STAT signaling pathway, >>Pathways in cancer
Also known as
IL13RA1; IL13R; IL13RA; Interleukin-13 receptor subunit alpha-1; IL-13 receptor subunit alpha-1; IL-13R subunit alpha-1; IL-13R-alpha-1; IL-13RA1; Cancer/testis antigen 19; CT19; CD antigen CD213a1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human IL-13R alpha1 around the phosphorylation site of Tyr405. AA range:371-420
Observed band
49kD
Cellular localization
Membrane; Single-pass type I membrane protein.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 371-420
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES5896
UniProt entry
P78552
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00