Manufactured by:ELK Biotechnology
RUO

CD36 rabbit pAb

CD36 rabbit pAb from ELK Biotechnology

SKU:
ABM108052
Product type
Primary Antibody
TargetCD36
ApplicationsWB, IHC, ELISA
Species / reactivityHuman, Mouse, Rat, Tilapia
Expression systemAdipocyte,Liver,Mammary gland,Milk,Placenta,Platelet,Skeletal muscle,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

CD36 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CD36 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat, Tilapia. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2014],

Additional antibody information

Specificity CD36 Polyclonal Antibody detects endogenous levels of CD36 protein.
Validation evidence captions Western Blot analysis of MCF7, 4T1 cells using CD36 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human Tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Key facts & specifications
Research areas
>>PPAR signaling pathway, >>Phagosome, >>AMPK signaling pathway, >>ECM-receptor interaction, >>Hematopoietic cell lineage, >>Adipocytokine signaling pathway, >>Insulin resistance, >>Fat digestion and absorption, >>Cholesterol metabolism, >>Malaria, >>Diabetic cardiomyopathy, >>Lipid and atherosclerosis
Also known as
CD36; GP3B; GP4; Platelet glycoprotein 4; Fatty acid translocase; FAT; Glycoprotein IIIb; GPIIIB; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; Platelet collagen receptor; Platelet glycoprotein IV; GPIV; Thrombospondin receptor; CD36
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human CD36. AA range:331-380
Observed band
90kD
Cellular localization
Cell membrane ; Multi-pass membrane protein . Membrane raft . Golgi apparatus . Apical cell membrane . Upon ligand-binding, internalized through dynamin-dependent endocytosis. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 331-380
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Tilapia
Identifiers & database references
Supplier SKU / catalogue number
ES4315
UniProt entry
P16671
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CD36CD36 molecule (CD36 blood group)

View target guide

CD36 identifies CD36 molecule (CD36 blood group) in Homo sapiens (Human). This target record connects NCBI Gene 948, UniProtKB P16671 and related ABMIUM catalogue products.

Biological function: CD36 (CD36 molecule; platelet glycoprotein 4/GPIV) is a multifunctional membrane glycoprotein and scavenger receptor that binds long-chain fatty acids and diverse lipid and protein ligands, linking lipid uptake with platelet, macrophage and vascular biology.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
948
UniProtKB
P16671
ELK Biotechnology
£220.00