Manufactured by:ELK Biotechnology
RUO

CD19 rabbit pAb

CD19 rabbit pAb from ELK Biotechnology

SKU:
ABM108049
Product type
Primary Antibody
TargetCD19
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemDetected on marginal zone and germinal center B cells in lymph nodes (PubMed:2463100). Detected on blood B cells (at protein level) (PubMed:2463100, PubMed:16672701).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

CD19 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CD19 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity CD19 Polyclonal Antibody detects endogenous levels of CD19 protein.
Validation evidence captions Western Blot analysis of mouse brain cells using CD19 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of MOUSE-BRAIN cells using CD19 Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-tonsils, antibody was diluted at 1:100
Key facts & specifications
Research areas
>>PI3K-Akt signaling pathway, >>Hematopoietic cell lineage, >>B cell receptor signaling pathway, >>Epstein-Barr virus infection, >>Primary immunodeficiency
Also known as
CD19; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000;Flow Cyt 1:50-200;IHC-p 1:100-500;IF(paraffin section);ELISA 1:5000-20000
Immunogen
Synthesized peptide derived from B-lymphocyte antigen CD19 at AA range: 191-240
Observed band
61kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein . Membrane raft ; Single-pass type I membrane protein .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;Flow Cyt 1:50-200;IHC-p 1:100-500;IF(paraffin section);ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 191-240
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES4310
UniProt entry
P15391
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CD19CD19 molecule

View target guide

CD19 molecule (CD19), also known as B-lymphocyte antigen CD19, is a B-cell surface glycoprotein and signalling coreceptor. This guide connects human NCBI Gene 930, UniProtKB P15391 and ABMIUM catalogue products associated with CD19.

Biological function: CD19 functions as a coreceptor for the B-cell antigen receptor complex, lowering the threshold for antigen-triggered B-cell activation. CD19 signalling can activate phosphatidylinositol 3-kinase pathways and mobilise intracellular calcium, supporting normal B-cell differentiation, proliferation and antibody responses.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
930
UniProtKB
P15391
ELK Biotechnology
£220.00