Manufactured by:ELK Biotechnology
RUO

SOD-2 rabbit pAb

SOD-2 rabbit pAb from ELK Biotechnology

SKU:
ABM108044
Product type
Primary Antibody
TargetSOD2
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemBrain,Colon,Heart,Liver,Lung,Mammary carcinoma,Tongue,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

SOD-2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying SOD2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016],

Additional antibody information

Specificity SOD-2 Polyclonal Antibody detects endogenous levels of SOD-2 protein.
Validation evidence captions Western Blot analysis of NIH-3T3 cells using SOD-2 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human lung cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Key facts & specifications
Research areas
>>FoxO signaling pathway, >>Peroxisome, >>Longevity regulating pathway, >>Longevity regulating pathway - multiple species, >>Huntington disease, >>Chemical carcinogenesis - reactive oxygen species, >>Lipid and atherosclerosis
Also known as
SOD2; Superoxide dismutase [Mn], mitochondrial
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human SOD2. AA range:91-140
Observed band
24kD
Cellular localization
Mitochondrion matrix.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 91-140
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES4305
UniProt entry
P04179
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SOD2superoxide dismutase 2

View target guide

SOD2 identifies superoxide dismutase 2 in Homo sapiens (Human). This target record connects NCBI Gene 6648, UniProtKB P04179 and related ABMIUM catalogue products.

Biological function: SOD2 is the mitochondrial manganese-dependent superoxide dismutase that converts superoxide radicals to hydrogen peroxide and molecular oxygen, providing a central mitochondrial antioxidant defense against reactive oxygen species.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6648
UniProtKB
P04179
ELK Biotechnology
£220.00