Manufactured by:ELK Biotechnology
RUO

SCCA1/2 rabbit pAb

SCCA1/2 rabbit pAb from ELK Biotechnology

SKU:
ABM107841
Product type
Primary Antibody
TargetSCCA1/2
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemSquamous cells. Expressed in some hepatocellular carcinoma (at protein level).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

SCCA1/2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying SCCA1/2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

developmental stage:Its expression is closely related to cellular differentiation in both normal and malignant squamous cells.,function:May act as a protease inhibitor to modulate the host immune response against tumor cells.,similarity:Belongs to the serpin family.,similarity:Belongs to the serpin family. Ov-serpin subfamily.,subcellular location:Seems to also be secreted in plasma by cancerous cells but at a low level.,tissue specificity:Squamous cells. Expressed in some hepatocellular carcinoma (at protein level).,

Additional antibody information

Specificity SCCA1/2 Polyclonal Antibody detects endogenous levels of SCCA1/2 protein.
Validation evidence captions Western Blot analysis of Jurkat, 293 cells using SCCA1/2 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:100 | Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
Key facts & specifications
Research areas
>>Amoebiasis
Also known as
SERPINB3; SCCA; SCCA1; Serpin B3; Protein T4-A; Squamous cell carcinoma antigen 1; SCCA-1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human SERPINB3/4. AA range:111-160
Observed band
45kD
Cellular localization
Cytoplasm . Seems to also be secreted in plasma by cancerous cells but at a low level.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 111-160
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES4040
UniProt entry
P29508
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00