Manufactured by:ELK Biotechnology
RUO

Ribosomal Protein S4Y1 rabbit pAb

Ribosomal Protein S4Y1 rabbit pAb from ELK Biotechnology

SKU:
ABM107492
Product type
Primary Antibody
TargetRibosomal Protein S4Y1
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemCoronary artery,Fetal liver,PCR rescued clones,Placenta,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Ribosomal Protein S4Y1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Ribosomal Protein S4Y1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, X-linked (RPS4X). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throug

Additional antibody information

Specificity Ribosomal Protein S4Y1 Polyclonal Antibody detects endogenous levels of Ribosomal Protein S4Y1 protein.
Validation evidence captions Western Blot analysis of various cells using Ribosomal Protein S4Y1 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of 293T mouse-spleen lysis using Ribosomal Protein S4Y1 antibody. Antibody was diluted at 1:1000 | Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Immunofluorescence analysis of HUVEC cells, using RPS4Y1 Antibody. The picture on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>Ribosome, >>Coronavirus disease - COVID-19
Also known as
RPS4Y1; RPS4Y; 40S ribosomal protein S4; Y isoform 1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human RPS4Y1. AA range:214-263
Observed band
29kD
Cellular localization
nucleus,nucleoplasm,cytosol,ribosome,polysome,membrane,cytosolic small ribosomal subunit,
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 214-263
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES3375
UniProt entry
P22090
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00