Flotillin-2 rabbit pAb

Flotillin-2 rabbit pAb

Flotillin-2 rabbit pAb
Manufactured by:ELK Biotechnology
RUO

Flotillin-2 rabbit pAb

Flotillin-2 rabbit pAb from ELK Biotechnology

SKU:
ABM107059
Product type
Primary Antibody
TargetFlotillin-2
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemIn skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Flotillin-2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Flotillin-2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Flotillin-2 Polyclonal Antibody detects endogenous levels of Flotillin-2 protein.
Validation evidence captions Western Blot analysis of various cells using Flotillin-2 Polyclonal Antibody | Western blot analysis of lysate from A549 cells, using Flotillin-2 antibody. | Western blot analysis of 1) A431, 2)Mouse Brain Tissue, 3) Rat Brain Tissue with Flotllin-2 Rabbit pAb diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma using Flotillin-2 Rabbit pAb diluted at 1:200
Key facts & specifications
Research areas
>>Insulin signaling pathway
Also known as
FLOT2; ESA1; M17S1; Flotillin-2; Epidermal surface antigen; ESA; Membrane component chromosome 17 surface marker 1
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human Flotillin-2. AA range:95-144
Observed band
46kD
Cellular localization
Cell membrane ; Peripheral membrane protein . Membrane, caveola ; Peripheral membrane protein . Endosome . Membrane ; Lipid-anchor . Membrane-associated protein of caveolae.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 95-144
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES2353
UniProt entry
Q14254
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00