Manufactured by:ELK Biotechnology
RUO

Endoplasmin rabbit pAb

Endoplasmin rabbit pAb from ELK Biotechnology

SKU:
ABM107020
Product type
Primary Antibody
TargetEndoplasmin
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse
Expression systemBlood,Brain,Liver,Lung,Platelet,Skin,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Endoplasmin rabbit pAb is a research-use antibody from ELK Biotechnology for studying Endoplasmin in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012],

Additional antibody information

Specificity Endoplasmin Polyclonal Antibody detects endogenous levels of Endoplasmin protein.
Validation evidence captions Western Blot analysis of various cells using Endoplasmin Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of HeLa cells using Endoplasmin Polyclonal Antibody diluted at 1:1000 | Immunofluorescence analysis of HepG2 cells, using GRP94 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using GRP94 Antibody. The picture on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>Protein processing in endoplasmic reticulum, >>PI3K-Akt signaling pathway, >>IL-17 signaling pathway, >>Estrogen signaling pathway, >>Thyroid hormone synthesis, >>Salmonella infection, >>Pathways in cancer, >>Chemical carcinogenesis - receptor activation, >>Prostate cancer, >>Lipid and atherosclerosis, >>Fluid shear stress and atherosclerosis
Also known as
HSP90B1; GRP94; TRA1; Endoplasmin; 94 kDa glucose-regulated protein; GRP-94; Heat shock protein 90 kDa beta member 1; Tumor rejection antigen 1; gp96 homolog
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human GRP94. AA range:754-803
Observed band
92kD
Cellular localization
Endoplasmic reticulum lumen . Sarcoplasmic reticulum lumen . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 754-803
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES2263
UniProt entry
P14625
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00