Manufactured by:ELK Biotechnology
RUO

CCKBR rabbit pAb

CCKBR rabbit pAb from ELK Biotechnology

SKU:
ABM106920
Product type
Primary Antibody
TargetCCKBR
ApplicationsWB
Species / reactivityHuman, Rat, Mouse
Expression systemIsoform 1 is expressed in brain, pancreas, stomach, the colon cancer cell line LoVo and the T-lymphoblastoma Jurkat, but not in heart, placenta, liver, lung, skeletal muscle, kidney or the stomach cancer cell line AGS. Expressed at high levels in the small cell lung cancer cell line NCI-H510, at lower levels in NCI-H345, NCI-H69 and GLC-28 cell lines, not expressed in GLC-19 cell line. Within the stomach, expressed at high levels in the mucosa of the gastric fundus and at low levels in the antrum and duodenum. Isoform 2 is present in pancreatic cancer cells and colorectal cancer cells, but not in normal pancreas or colonic mucosa. Isoform 3 is expressed in brain, pancreas, stomach, the stomach cancer cell line AGS and the colon cancer cell line LoVo.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

CCKBR rabbit pAb is a research-use antibody from ELK Biotechnology for studying CCKBR in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. Alternative splicing results in multiple transcript variants. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors. [provided by RefSeq, Dec 2015],

Additional antibody information

Specificity CCKBR protein(A214) detects endogenous levels of CCKBR
Validation evidence captions Western blot analysis of 1) Mouse Brain Tissue, 2)Rat Brain Tissue, 3)Human Brain Tissue, with CCKBR Rabbit pAb diluted at 1:2,000.
Key facts & specifications
Research areas
>>Calcium signaling pathway, >>Neuroactive ligand-receptor interaction, >>Gastric acid secretion
Also known as
CCKBR; CCKRB; Gastrin/cholecystokinin type B receptor; CCK-B receptor; CCK-BR; Cholecystokinin-2 receptor; CCK2-R
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:1000-2000
Immunogen
Synthetic Peptide of CCKBR AA range: 306-356
Observed band
50kD
Cellular localization
Cell membrane; Multi-pass membrane protein.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 306-356
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES20790
UniProt entry
P32239
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00