GLSK rabbit pAb 50µL/100µL
Manufactured by:ELK Biotechnology
RUO

GLSK rabbit pAb

GLSK rabbit pAb from ELK Biotechnology

SKU:
ABM105658
Product type
Primary Antibody
TargetGLSK
ApplicationsWB
Species / reactivityHuman, Mouse, Rat
Expression systemIsoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

GLSK rabbit pAb is a research-use antibody from ELK Biotechnology for studying GLSK in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012],

Additional antibody information

Specificity This antibody detects endogenous levels of GLSK at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
>>Arginine biosynthesis, >>Alanine, aspartate and glutamate metabolism, >>D-Amino acid metabolism, >>Metabolic pathways, >>Glutamatergic synapse, >>GABAergic synapse, >>Proximal tubule bicarbonate reclamation, >>MicroRNAs in cancer, >>Central carbon metabolism in cancer
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1：500-2000
Immunogen
Synthesized peptide derived from human GLSK AA range: 162-212
Cellular localization
[Isoform 1]: Mitochondrion . Cytoplasm, cytosol . The 74-kDa cytosolic precursor is translocated into the mitochondria and processed via a 72-kDa intermediate to yield the mature 68- and 65-kDa subunits. .; [Isoform 3]: Mitochondrion .; [Glutaminase kidney isoform, mitochondrial 68 kDa chain]: Mitochondrion matrix . Produced by the proteolytic processing of the 74-kDa cytosolic precursor. .; [Glutaminase kidney isoform, mitochondrial 65 kDa chain]: Mitochondrion matrix . Produced by the proteolytic processing of the 74-kDa cytosolic precursor. .
Assay type
Antibody validation/application
Source organism
Rabbit
Molecular weight
74kD
Concentration
1 mg/ml
Working concentration
WB 1：500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 162-212
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES16173
UniProt entry
O94925
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00