Manufactured by:ELK Biotechnology
RUO

JAK2 (phospho Tyr1007) rabbit pAb

JAK2 (phospho Tyr1007) rabbit pAb from ELK Biotechnology

SKU:
ABM104617
Product type
Primary Antibody
TargetJAK2
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemUbiquitously expressed throughout most tissues.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

JAK2 (phospho Tyr1007) rabbit pAb is a research-use antibody from ELK Biotechnology for studying JAK2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Phospho-JAK2 (Y1007) Polyclonal Antibody detects endogenous levels of JAK2 protein only when phosphorylated at Y1007.
Validation evidence captions Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,JAK2 (phospho Tyr1007) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,JAK2 (phospho Tyr1007) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,JAK2 (phospho Tyr1007) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only. | Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,JAK2 (phospho Tyr1007) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Key facts & specifications
Research areas
>>EGFR tyrosine kinase inhibitor resistance, >>Chemokine signaling pathway, >>PI3K-Akt signaling pathway, >>Necroptosis, >>Signaling pathways regulating pluripotency of stem cells, >>JAK-STAT signaling pathway, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>Cholinergic synapse, >>Prolactin signaling pathway, >>Adipocytokine signaling pathway, >>AGE-RAGE signaling pathway in diabetic complications, >>Growth hormone synthesis, secretion and action, >>Leishmaniasis, >>Toxoplasmosis, >>Tuberculosis, >>Hepatitis B, >>Influenza A, >>Kaposi sarcoma-associated herpesvirus infection, >>Herpes simplex virus 1 infection, >>Pathways in cancer, >>Chemical carcinogenesis - receptor activation, >>PD-L1 expression and PD-1 checkpoint pathway in cancer, >>Lipid and atherosclerosis
Also known as
JAK2; Tyrosine-protein kinase JAK2; Janus kinase 2; JAK-2
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human JAK2 around the phosphorylation site of Tyr1007. AA range:981-1030
Observed band
130kD
Cellular localization
Endomembrane system ; Peripheral membrane protein . Cytoplasm . Nucleus .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 981-1030
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES1348
UniProt entry
O60674
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

JAK2Janus kinase 2

View target guide

JAK2 identifies Janus kinase 2 in Homo sapiens (Human). This target record connects NCBI Gene 3717, UniProtKB O60674 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3717
UniProtKB
O60674
ELK Biotechnology
£220.00