IKKα/β (phospho Ser180/181) rabbit pAb

IKKα/β (phospho Ser180/181) rabbit pAb

IKKα/β (phospho Ser180/181) rabbit pAb
Manufactured by:ELK Biotechnology
RUO

IKKα/β (phospho Ser180/181) rabbit pAb

IKKα/β (phospho Ser180/181) rabbit pAb from ELK Biotechnology

SKU:
ABM104543
Product type
Primary Antibody
TargetIKKα/β
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemWidely expressed.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

IKKα/β (phospho Ser180/181) rabbit pAb is a research-use antibody from ELK Biotechnology for studying IKKα/β in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Phospho-IKKα/β (S180/181) Polyclonal Antibody detects endogenous levels of IKKα/β protein only when phosphorylated at S180/181.
Validation evidence captions Western Blot analysis of various cells using Phospho-IKKα/β (S180/181) Polyclonal Antibody diluted at 1:1000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IKK-alpha/beta (Phospho-Ser180/181) Antibody | Immunohistochemistry analysis of paraffin-embedded human heart, using IKK-alpha/beta (Phospho-Ser180/181) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HepG2 cells treated with TNF 20ng/ml 5', using IKK-alpha/beta (Phospho-Ser180/181) Antibody. The lane on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Antifolate resistance, >>MAPK signaling pathway, >>Ras signaling pathway, >>Chemokine signaling pathway, >>NF-kappa B signaling pathway, >>FoxO signaling pathway, >>mTOR signaling pathway, >>PI3K-Akt signaling pathway, >>Apoptosis, >>Osteoclast differentiation, >>Toll-like receptor signaling pathway, >>NOD-like receptor signaling pathway, >>RIG-I-like receptor signaling pathway, >>Cytosolic DNA-sensing pathway, >>C-type lectin receptor signaling pathway, >>IL-17 signaling pathway, >>Th1 and Th2 cell differentiation, >>Th17 cell differentiation, >>T cell receptor signaling pathway, >>B cell receptor signaling pathway, >>TNF signaling pathway, >>Adipocytokine signaling pathway, >>Alcoholic liver disease, >>Alzheimer disease, >>Epithelial cell signaling in Helicobacter pylori infection, >>Pathogenic Escherichia coli infection, >>Shigellosis, >>Salmonella infection, >>Yersinia infection, >>Chagas disease, >>Toxoplasmosis, >>Hepatitis C, >>Hepatitis B, >>Measles, >>Human cytomegalovirus infection, >>Influenza A, >>Human pap
Also known as
CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human IKK-alpha/beta around the phosphorylation site of Ser180/181. AA range:151-200
Observed band
85kD
Cellular localization
Cytoplasm . Nucleus . Shuttles between the cytoplasm and the nucleus.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 151-200
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES1337
UniProt entry
O15111/O14920
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00