Manufactured by:ELK Biotechnology
RUO

Recombinant E.coli Trp synthase (N-6His)

Recombinant E.coli Trp synthase (N-6His) from ELK Biotechnology

SKU:
ABM100958
Product type
Recombinant Proteins
TargetRecombinant E.coli Trp synthase
ApplicationsProtein research
Purity>95% by SDS-PAGE

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Recombinant E.coli Trp synthase (N-6His) is a research-use recombinant protein from ELK Biotechnology preparation representing Recombinant E.coli Trp synthase supplied as Lyophilized. Review the expression, purity, formulation and storage specifications below before selecting it for an assay or functional workflow.

Description

Recombinant E.coli Tryptophan Synthase is produced by our E.coli expression system and the target gene encoding Met1-Ser268&Thr2-Ile397 is expressed with a 6His tag at the N-terminus.

Background

Tryptophan synthase is a multienzyme α2β2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two steps in the synthesis of L-tryptophan (L-Trp). The α-subunit catalyzes cleavage of 3-indole-d-glycerol 3′-phosphate (IGP) to give indole and D-glyceraldehyde 3′-phosphate (G3P). Indole is then transferred through a 25-Å hydrophobic tunnel to the β-subunit. The β2 subunit contains pyridoxal 5'-phosphate and catalyzes several pyridoxal 5'-phosphate-dependent reactions, including/3-elimination reactions 6 and a thiol-dependent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target.

Handling information

Delivery condition The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Key facts & specifications
Research areas
Molecular Biology
Also known as
Tryptophan synthetase; Tryptophan synthase
Assay type
Recombinant protein
Molecular weight
28.7&43.8 KDa
Form / buffer
Lyophilized
Endotoxin level
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Quantity per pack
50ug
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpecificationPurityEndotoxinBioactivity
Product QC
Identifiers & database references
Supplier SKU / catalogue number
EPT234
UniProt entry
P0A877&P0A879
Storage, stability & shipping
Storage conditions
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Long-term storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£523.00