Manufactured by:ELK Biotechnology
RUO

Human AIMP1(Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1) ELISA Kit

Human AIMP1(Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1) ELISA Kit from ELK Biotechnology

SKU:
ABM72698
Product type
ELISA Kits
TargetAIMP1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Human AIMP1(Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring AIMP1 in Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AIMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AIMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AIMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AIMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include SCYE1; EMAP II; EMAP2; p43; Small Inducible Cytokine Subfamily E,Member 1; Endothelial monocyte-activating polypeptide II; Multisynthase complex auxiliary component p43.

Key facts & specifications
Research areas
Enzyme & Kinase
Also known as
SCYE1; EMAP II; EMAP2; p43; Small Inducible Cytokine Subfamily E,Member 1; Endothelial monocyte-activating polypeptide II; Multisynthase complex auxiliary component p43
Assay type
Sandwich
Detection method
Colorimetric (TMB/HRP)
Sensitivity
43 pg/mL
Detection range
125-8000 pg/mL
Plate format
96-well pre-coated
Form / buffer
Pre-coated microplate kit
Quantity per pack
48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typeSerumPlasmaTissue homogenatesCell lysatesCell culture supernatantsOther biological fluids
Sample compatibility
Identifiers & database references
Supplier SKU / catalogue number
ELK6321
UniProt entry
Q12904
Storage, stability & shipping
Storage conditions
Store according to the conditions stated in the product manual.
Long-term storage
Store according to the conditions stated in the product manual.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

AIMP1aminoacyl tRNA synthetase complex interacting multifunctional protein 1

View target guide

AIMP1 (aminoacyl tRNA synthetase complex interacting multifunctional protein 1; p43/EMAPII) is a multisynthetase-complex protein studied in tRNA handling, signalling and extracellular cytokine-like biology.

Biological function: AIMP1 is a non-catalytic component of the cytoplasmic multisynthetase complex that binds tRNA and supports aminoacyl-tRNA synthetase activity. Reviewed UniProt also describes signalling functions, including inflammatory cytokine activity and regulation of TGF-beta signalling through SMURF2 stabilization.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
9255
UniProtKB
Q12904
ELK Biotechnology
£284.00