Manufactured by:ELK Biotechnology
RUO

A Cyclase IV rabbit pAb

A Cyclase IV rabbit pAb from ELK Biotechnology

SKU:
ABM108426
Product type
Primary Antibody
TargetA Cyclase IV
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemDetected in the zona glomerulosa and the zona fasciculata in the adrenal gland (at protein level).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

A Cyclase IV rabbit pAb is a research-use antibody from ELK Biotechnology for studying A Cyclase IV in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the family of adenylate cyclases, which are membrane-associated enzymes that catalyze the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). Mouse studies show that adenylate cyclase 4, along with adenylate cyclases 2 and 3, is expressed in olfactory cilia, suggesting that several different adenylate cyclases may couple to olfactory receptors and that there may be multiple receptor-mediated mechanisms for the generation of cAMP signals. Alternative splicing results in transcript variants. [provided by RefSeq, Nov 2010],

Additional antibody information

Specificity A Cyclase IV Polyclonal Antibody detects endogenous levels of A Cyclase IV protein.
Validation evidence captions Western Blot analysis of Jurkat cells using A Cyclase IV Polyclonal Antibody | Western blot analysis of lysates from Jurkat cells, using ADCY4 Antibody. The lane on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>Purine metabolism, >>Metabolic pathways, >>Endocrine resistance, >>Rap1 signaling pathway, >>Calcium signaling pathway, >>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>Phospholipase D signaling pathway, >>Oocyte meiosis, >>Longevity regulating pathway, >>Longevity regulating pathway - multiple species, >>Adrenergic signaling in cardiomyocytes, >>Vascular smooth muscle contraction, >>Apelin signaling pathway, >>Gap junction, >>Platelet activation, >>Circadian entrainment, >>Thermogenesis, >>Retrograde endocannabinoid signaling, >>Glutamatergic synapse, >>Cholinergic synapse, >>GABAergic synapse, >>Taste transduction, >>Inflammatory mediator regulation of TRP channels, >>Insulin secretion, >>GnRH signaling pathway, >>Ovarian steroidogenesis, >>Progesterone-mediated oocyte maturation, >>Estrogen signaling pathway, >>Melanogenesis, >>Thyroid hormone synthesis, >>Oxytocin signaling pathway, >>Regulation of lipolysis in adipocytes, >>Aldosterone synthesis and secretion, >>Relaxin sig
Also known as
ADCY4; Adenylate cyclase type 4; ATP pyrophosphate-lyase 4; Adenylate cyclase type IV; Adenylyl cyclase 4
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human ADCY4. AA range:195-244
Observed band
120kD
Cellular localization
Cell membrane ; Multi-pass membrane protein . Cytoplasm .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 195-244
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES5070
UniProt entry
Q8NFM4
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00