Manufactured by:Elabscience
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Human Bax (Bcl-2 Associated X Protein) CLIA Kit

Human Bax (Bcl-2 Associated X Protein) CLIA Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM10943-1
Product type
Assay Kits
TargetBAX

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Human Bax (Bcl-2 Associated X Protein) CLIA Kit from Elabscience is intended for research workflows involving BAX. Review the listed specifications and supporting documents to confirm compatibility with the intended method.

Key facts & specifications
Research areas
Cancer, Cell Biology, Metabolism
Also known as
BCL2L4
Cross-reactivity
This kit recognizes Human BAX in samples.No significant cross-reactivity or interference between Human BAX and analogues was observed
Incubation temperature
2-8℃/-20℃
Assay type
CLIA Kits
Detection method
Chemiluminescence
Plate format
48-well microplate plates pre-coated with anti-BAX capture antibody
Technical note
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BAX. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BAX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BAX, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human BAX. You can calculate the concentration of Human BAX in the samples by comparing the RLU value of the samples to the standard curve.
Identifiers & database references
Supplier SKU / catalogue number
E-CL-H0440_48T
Gene symbol
BAX
Gene ID
581
UniProt accession
Q07812
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

BAXBCL2 associated X, apoptosis regulator

View target guide

BAX (BCL2 associated X, apoptosis regulator; apoptosis regulator BAX) is a pro-apoptotic BCL-2 family protein that controls mitochondrial outer-membrane permeabilization during intrinsic apoptosis. This catalogue-backed target record connects verified human BAX identity with Abmium research reagents.

Biological function: BAX is a pro-apoptotic BCL-2 family effector. Under cellular stress it undergoes activating conformational changes, accumulates at the mitochondrial outer membrane and promotes membrane permeabilization, enabling cytochrome c release and downstream apoptotic signalling. Its activity is regulated within the wider balance of pro- and anti-apoptotic BCL-2 family proteins.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
581
UniProtKB
Q07812
Elabscience
£407.00£325.60