Manufactured by:Boster Bio
ABMIUM Verified™RUO

Human SPARC ELISA Kit PicoKine®

ELISA Kit supplied by Boster Bio

SKU:
ABM32823
Product type
ELISA Kits
TargetSPARC
ApplicationsELISA
Species / reactivityHuman
Sample typeCell culture supernatant, serum and human milk.
PurityNatural and recombinant human SPARC

Manufacturer provenance

Manufactured by:
Boster Bio
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Human SPARC ELISA Kit PicoKine® is a research-use ELISA kit from Boster Bio for measuring SPARC in Cell culture supernatant, serum and human milk. samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Human SPARC ELISA Kit PicoKine® (96 Tests). Quantitate Human SPARC in cell culture supernatants, serum and human milk. Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

SPARC (secreted protein acidic and rich in cysteine), also known as Osteonectin, is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is a glycoprotein of 40 kD. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Osteonectin is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found.

Product information

Key facts & specifications
Also known as
Basement membrane protein 40; BM 40; ON; Osteonectin; SPARC
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: NS0; Immunogen sequence: A18-I303
Sensitivity
<20 pg/ml
Detection range
0.78 ng/ml - 50 ng/ml
Sequence fragment
Expression system for standard: NS0; Immunogen sequence: A18-I303
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typeSample CompatibilityAssay RangeSensitivitySpecificity
cell culture supernatants
serum and human milk.
Identifiers & database references
Supplier SKU / catalogue number
EK1210
Gene symbol
SPARC
Gene ID
6678
UniProt accession
P09486
Storage, stability & shipping
Shelf life
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Storage conditions
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Long-term storage
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Compliance & documents
CE marked
No
Datasheet
Open datasheet
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Boster Bio and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SPARCsecreted protein acidic and cysteine rich

View target guide

SPARC identifies secreted protein acidic and cysteine rich in Homo sapiens (Human). This target record connects NCBI Gene 6678, UniProtKB P09486 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
6678
UniProtKB
P09486
Boster Bio
£442.00