Manufactured by:ELK Biotechnology
RUO

CD28 (phospho Tyr218) rabbit pAb

CD28 (phospho Tyr218) rabbit pAb from ELK Biotechnology

SKU:
ABM109567
Product type
Primary Antibody
TargetCD28
ApplicationsWB, ELISA
Species / reactivityHuman
Expression systemExpressed in T-cells and plasma cells, but not in less mature B-cells.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

CD28 (phospho Tyr218) rabbit pAb is a research-use antibody from ELK Biotechnology for studying CD28 in WB, ELISA workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011],

Additional antibody information

Specificity Phospho-CD28 (Y218) Polyclonal Antibody detects endogenous levels of CD28 protein only when phosphorylated at Y218.
Validation evidence captions Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD28 (Phospho-Tyr218) Antibody | Western blot analysis of lysates from HepG2 cells treated with nocodazole 1ug/ml 16h, using CD28 (Phospho-Tyr218) Antibody. The lane on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Cell adhesion molecules, >>T cell receptor signaling pathway, >>Intestinal immune network for IgA production, >>Type I diabetes mellitus, >>Measles, >>PD-L1 expression and PD-1 checkpoint pathway in cancer, >>Autoimmune thyroid disease, >>Systemic lupus erythematosus, >>Rheumatoid arthritis, >>Allograft rejection, >>Graft-versus-host disease, >>Viral myocarditis
Also known as
CD28; T-cell-specific surface glycoprotein CD28; TP44; CD antigen CD28
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218. AA range:171-220
Observed band
45kD
Cellular localization
Membrane; Single-pass type I membrane protein.; [Isoform 3]: Cell surface .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 171-220
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Identifiers & database references
Supplier SKU / catalogue number
ES8032
UniProt entry
P10747
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CD28CD28 molecule

View target guide

CD28 identifies CD28 molecule in Homo sapiens (Human). This target record connects NCBI Gene 940, UniProtKB P10747 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
940
UniProtKB
P10747
ELK Biotechnology
£220.00